Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: Schematic representation of plasmonic fiberoptic absorbance biosensor (P-FAB) strategy for the detection of SARS-CoV-2 N-protein, photographic image oof GOF and POF sensor probes, and the LED-PD based experimental set-up used for the optical absorbance measurement.

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques:

Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: Dose-response curve obtained from POF sensor probes with capture antibody (Anti-SARS-CoV-2 N-protein IgG1) immobilized by means of physisorption, Acid-treatment, HMDA, and EDC/NHS based covalent binding methodology using a green LED-photodetector setup due to binding of immunocomplex with AuNP labels (40 nm, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$10\times $ \end{document} ) conjugated with anti-SARS CoV-2 -N-protein IgG2 (n = 3).

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques: Binding Assay

Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: Absorbance response obtained for a range of SARS-CoV-2 N-protein concentrations from POF and GOF sensor probes chemisorbed with anti-SARS-CoV-2 -N-protein IgG1 with the help of a green LED-photodetector setup due to binding of immunocomplex with AuNP labels (40 nm, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$10\times $ \end{document} ) conjugated with anti-SARS CoV-2 -N-protein IgG2 (n = 3). The width of the control band represents the standard deviation of respective POF and GOF sensor probes (n = 3).

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques: Binding Assay, Standard Deviation

Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: (A) Photographic images of AuNP conjugates of varying sizes subjected to NaCl stability test. Stable conjugates remain pink, while the AuNP labels with insufficient surface coverage agglomerates and turns violet or colorless. (B) Schematic representation showing the possible orientation of IgG on the AuNP surface. (C) Absorbance spectra of AuNP labels for varying sizes obtained using USB 4000 spectrometer. A broader full-width half maxima of the citrate-capped AuNPs indicates a 30-40% wider size distribution in comparison to 40 nm AuNP.

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques:

Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: (A) Representative temporal absorbance response and (B) dose-response curves obtained for a range of SARS-CoV-2 N-protein concentrations from GOF sensor probes chemisorbed with anti-SARS-CoV-2-N-protein IgG1 with the help of the optical experimental setup due to binding of immunocomplex with AuNP labels varying sizes and \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$10\times $ \end{document} concentration conjugated with anti-SARS CoV-2 -N-protein IgG2 (n = 3). Inset: TEM images of citrate reduced AuNPs prepared in-house.

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques: Binding Assay, Concentration Assay

Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: Normalized absorbance response obtained from U-bent GOF sensor probes immobilized with anti-SARS CoV-2 IgG1 and * goat anti-HIgG due to specific and non-specific binding (NSB) of anti-SARS CoV-2 N-protein IgG2-AuNP labels with at varying concentrations (n = 3), respectively. A (C) refers to the average absorbance response obtained from the control experiments for NSB, while A (T) refers to the absorbance response obtained from each of the analyte concentrations (specific or non-specific).

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques: Binding Assay

Journal: Cell Communication and Signaling : CCS

Article Title: Subsequent malaria enhances virus-specific T cell immunity in SIV-infected Chinese rhesus macaques

doi: 10.1186/s12964-022-00910-7

Figure Lengend Snippet: T-cell repertoire diversity and SIV-specific immune response in malaria- and SIV-infected monkeys. A Diversity index differences with respect to the 8th week of SIV infection (corresponding to malaria introduction in the S + P group). Repertoire diversity was restored in the S + P group but not in the P + S group. A significant difference between the S + P and P + S groups was observed for the Shannon diversity index. P -values computed using ANOVA. B Ratio of SIV-specific clonotype frequency with respect to the control point (8th week of SIV infection, corresponding to malaria introduction in the S + P group). The S + P group, but not the P + S group, was characterized by a persistently higher SIV-specific clonotype frequency. P -values were calculated using ANOVA. C New SIV-specific clonotype production. Fractions of existing (detected at previous sampling point) and new SIV-specific clonotypes shown for each monkey in the P + S, S and S + P groups. A significant increase in new SIV-specific clonotype production was observed in the S + P group during the whole period of Plasmodium infection (at 11–17 weeks and 50 weeks post SIV infection). D Frequency of IFN-g-producing SIV Gag-specific PBMCs. Number of IFN-γ-producing PBMCs against pooled SIV Gag peptides analyzed by ELISPOT. The sample sizes were 6, 4 and 5 on day 49 and 4, 3, and 5 on day 161 of the S, P + S and S + P groups, respectively. SFC: spot-forming cells

Article Snippet: The plates were washed with PBST (PBS containing 0.05% Tween-20), and a biotinylated anti-IFN-γ polyclonal detector antibody (BD Biosciences) was added.

Techniques: Infection, Sampling, Enzyme-linked Immunospot

Journal: Frontiers in Immunology

Article Title: Immune Protection of SIV Challenge by PD-1 Blockade During Vaccination in Rhesus Monkeys

doi: 10.3389/fimmu.2018.02415

Figure Lengend Snippet: Modulating SIV-specific T cell immune responses by PD-1 blockade in vitro . SIV-specific T cells from chronic SIV-infected monkeys were stimulated with either SIV peptide pool alone or with SIV peptides in combination with genolimzumab, and then detected by IFN-γ-mediated enzyme-linked immunospot (ELISPOT) assay (A) . The above samples were also analyzed for IFN-γ expression (B) and IL-2 (C) expression by Q-PCR. Moreover, the frequency of SIV Gag-specific cytokine-secreting CD3+ T cells was further detected by intracellular cytokine staining. IFN-γ+ CD3+ T cells (D) , IL-2+CD3 T cells (E) , and TNF-α+CD3+ T cells (F) are indicated, respectively. The final data are represented as the mean ± SEM from at least triplicate experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: SIV peptide pools were added into cells for 20–24 h for stimulation, and then a polyclonal anti-monkey IFN-γ biotinylated detector antibody (BD Pharmingen) was added.

Techniques: In Vitro, Infection, Enzyme-linked Immunospot, Expressing, Staining

Journal: Frontiers in Immunology

Article Title: Immune Protection of SIV Challenge by PD-1 Blockade During Vaccination in Rhesus Monkeys

doi: 10.3389/fimmu.2018.02415

Figure Lengend Snippet: SIV-specific T cell immune responses regulated by PD-1 blockade in vivo in rhesus monkeys. Rhesus monkeys were intravenously injected with anti-PD-1 antibody during the SIV vaccine immunization schedule as described in Figure . For assessing total cellular immune responses induced by SIV vaccine regimen, IFN-γ-secreting cells were detected by ELISPOT assay over the course of immunization. (A) Representative data to illustrate IFN-γ-mediated ELISPOT cellular immune responses against SIV structure proteins (Gag, Pol, and Env), for each monkey at 9 weeks after first immunization. (B) Dynamic analysis of SIV-specific IFN-γ-mediated ELISPOT cellular immune responses over time until 42 weeks after first immunization. Data represent spot-forming cells (SFC) per million PBMCs. For assessing the polyfunctionality of SIV-specific CD8+ T cells, IFN-γ-and TNF-α-secreting cells were detected by intracellular cytokine staining (ICS) assays. (C) Representative flow cytometry dot plots for IFN-γ-and TNF-α-secreting CD8+ T cell populations. (D) Statistical analysis for the frequency of IFN-γ-and TNF-α-secreting CD8+ T cells by ICS. CFSE staining was used for assessing the ability of SIV antigen-stimulated T cell proliferation, whereby PBMCs were incubated with CFSE stain and stimulated with SIV peptides. (E) Representative dot plots for specific CD8+ T cell proliferation by CFSE staining. Statistical analysis for the proliferation index of CD8+ T cells in response to SIV antigen stimulation (F) or PMA+I simulation (G) . The daughter cells from proliferation had lower fluorescence intensity (CFSE-low cells). DMSO treatment was used as a negative control. * P < 0.05; *** P < 0.001.

Article Snippet: SIV peptide pools were added into cells for 20–24 h for stimulation, and then a polyclonal anti-monkey IFN-γ biotinylated detector antibody (BD Pharmingen) was added.

Techniques: In Vivo, Injection, Enzyme-linked Immunospot, Staining, Flow Cytometry, Incubation, Fluorescence, Negative Control